Figure 4.

Effect of SB220025 on iNOS mRNA degradation. Cells were stimulated with LPS (10 ng/ml) 1 h before addition of SB220025 (0,5 μM). Actinomycin D (actD) (0,5 μg/ml) was added to cells 6 h after LPS. Incubations were terminated at the indicated time points after LPS and total RNA was isolated. iNOS and GAPDH mRNA was measured by real time RT-PCR. iNOS mRNA levels were normalized against GAPDH. Values are mean ± S.E.M. (n = 3).