Figure 2.

The effect of SB220025 on NO production when added before or at various time points after LPS (A) and time course of p38 MAPK activation following LPS (B). (A) Cells were treated with SB220025 1 h before or at indicated time points after stimulation with LPS. After 24 h incubation, the nitrite concentrations were measured as a marker of NO production. Values are mean ± S.E.M. (n = 6). **P < 0.01, ***P < 0.001 compared with cells treated with LPS only. (B) Cells were stimulated with LPS (10 ng/ml) and incubations were terminated at indicated time points. Parallel immunoblots were run from same cell lysates using antibodies against phosphorylated p38 (pp38) and total p38. The result is representative of three experiments with similar results.