Table 2

Characterization of compounds by heterologous competition binding

Compound
pKi



hD1
hD2L
hD3
hD4.4
hD5
h5HT2A

Haloperidol
6.55 ± 0.09
8.56 ± 0.05
8.00 ± 0.05
8.10 ± 0.04
7.50 ± 0.06
6.84 ± 0.12
Clozapine
6.68 ± 0.03
6.60 ± 0.06
6.13 ± 0.05
6.93 ± 0.08
6.50 ± 0.08
8.23 ± 0.07
LE300
7.98 ± 0.06
7.19 ± 0.04
6.48 ± 0.04
6.46 ± 0.08
7.99 ± 0.05
9.65 ± 0.04
LE400
5.58 ± 0.16
5.90 ± 0.05
5.28 ± 0.07
4.79 ± 0.06
5.44 ± 0.07
6.86 ± 0.13
LE401
4.77 ± 0.25
5.06 ± 0.13
4.83 ± 0.16
< 4a)
4.79 ± 0.50
< 4a)
LE403
7.94 ± 0.06
6.43 ± 0.07
6.14 ± 0.10
6.26 ± 0.06
7.84 ± 0.05
8.40 ± 0.08
LE404
8.47 ± 0.10
7.10 ± 0.05
6.73 ± 0.06
7.23 ± 0.03
8.53 ± 0.09
8.79 ± 0.07
LE410
7.76 ± 0.04
7.54 ± 0.06
6.86 ± 0.07
6.32 ± 0.06
7.78 ± 0.10
8.40 ± 0.10
LE420
6.89 ± 0.07
6.64 ± 0.05
6.07 ± 0.06
5.83 ± 0.11
6.92 ± 0.04
7.97 ± 0.05

a) Displacement of radioligand was < 30% at 10 μM

Haloperidol, clozapine, and LE compounds were characterized at dopamine and h5HT2A receptors. [3H]SCH23390 was used for hD1-like and [3H]spiperone for hD2-like and h5HT2A receptors. Displayed are pKi values ± SEM, n ≥ 3.

Hamacher et al. BMC Pharmacology 2006 6:11   doi:10.1186/1471-2210-6-11