Table 2

Bronchoalveolar lavage inflammation in BALB/c mice 24 hours after the challenge with 0.3, 0.5 or 1 mg/kg of LPS.

PBS (50 μl)

LPS (0.3 mg/kg)

LPS (0.5 mg/kg)

LPS (1 mg/kg)

Neutrophils (× 10⁵ cells/ml)

0.66 ± 0.21

9.9 ± 1.1^*

10.1 ± 0.8^*

11.6 ± 2.4^*

Macrophages (× 10⁵ cells/ml)

1.65 ± 0.23

1.24 ± 0.4

1.43 ± 0.07

1.69 ± 0.21

Lymphocytes (× 10⁵ cells/ml)

0.02 ± 0.01

0.10 ± 0.02^*

0.06 ± 0.01^*

0.10 ± 0.07^*

Total cell counts (× 10⁵ cells/ml)

2.34 ± 0.29

10.29 ± 1.09^*

11.6 ± 0.8^*

13.4 ± 2.6^*

MPO (mU/ml)

41.2 ± 0.007

1045.5 ± 178.6^*

1451.7 ± 123.4^*

1235.8 ± 368.7^*

Elastase (ng/ml)

0 ± 0

264.4 ± 21.9^*

215.8 ± 8.5^*

287.5 ± 21.4^*

Animals were intranasally challenged with 0.3, 0.5 or 1 mg/kg of LPS or PBS alone (50 μl). Twenty four hours after the challenge, animals were sacrificed and bronchoalveolar lavage performed. Data are expressed as mean ± SEM 6 mice per group. Significance, (P < 0.05) indicated by (*) is versus PBS-challenged mice.

Corteling et al. BMC Pharmacology 2002 2:1   doi:10.1186/1471-2210-2-1