Table 1

PCR amplification primers.

Target gene

Primer sequences

Ta

(°C)


FKBP5

Fwd 5'-CCCTCGAATGCAACTCTCTT-3'

Rev 5'-TCTCCTTTCCGTTTGGTTCT-3'

62


GAPDH

Fwd 5'-TGCACCACCAACTGCTTAG-3'

Rev 5'-GATGCAGATGATGATGTTC-3'

57


IL-12p35

Fwd 5'-CATGCTGGCAGTTATTGA-3'

Rev 5'-AAGTATGCAGAGCTTGATTTT-3'

57


IL-12p40

Fwd 5'-AGGAGAGTCTGCCCATTGAGG-3'

Rev 5'-GACCTCCACCTGCCGAGAA-3'

65


IL-23p19

Fwd 5'-CGTCTCCTTCTCCGCTTCA-3'

Rev 5'-GTGCCTGGGGTGGTAGATTT-3'

65


MKP-1

Fwd 5'-AGCAGAGGCGAAGCATCATC-3'

Rev 5'-CCCAGCCTCTGCCGAAC-3'

61


a) Fwd: forward primer.

b) Rev: reverse primer.

c) Ta: annealing temperature.

d) GAPDH: internal reference gene.

Palma et al. BMC Pharmacology 2011 11:8   doi:10.1186/1471-2210-11-8

Open Data