|
Sequence of the marked CNBr fragments in figures 5 and 6. MW: molecular weight; AA: amino acid; the several amino acids are shown in the one-letter-code. Sequenced fragments of the α1-subunit are signed as in Tab. 4, while CNBr fragments of the β1-subunit are defined by the position of the determined AA in the subunit. The CNBr-2 fragment of the α1-subunit, represented in all labeled bands, |
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| CNBr-fragment |
α1-subunit |
β1-subunit |
Sequence from the N-terminus |
MW [kDa] |
|
|
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| CNBr I |
CNBr -1 to 8 |
APGQVPTEPIEE... |
51.8 |
|
| CNBr II |
CNBr -1 to 4 |
APGQVPTEPIEE... |
35.9 |
|
| CNBr III |
CNBr -1 and 2 |
APGQVPTEPIEE... |
30.2 |
|
| CNBr IV |
CNBr 16 |
PRYCLFGNNVT... |
11.2 |
|
| CNBr 8 |
IYIVESSAILFLG... |
12.5 |
||
| CNBr -2 and 3 |
PPCFRSECTEF... |
11.7 |
||
| AA 505-619 |
EIAGQVQVDGE... |
12.8 |
||
| CNBr V |
CNBr -10 to 13 |
LNALYTRFDQQ... |
6.5 |
|
| CNBr 3 |
LDRDLAILQLG... |
5.5 |
||
| CNBr 2 |
PPCFRSECTEF... |
6.2 |
||
| AA 2-69 |
YGFVNHALELL... |
7.7 |
||
| AA 116-164 |
RAPSFRCTDAE... |
5.5 |
||
| AA 445-503 |
KIVNLLNDLYTR... |
6.7 |
||
| AA 538-592 |
PRYCLFGNTVN... |
6.2 |
||
| CNBr VI |
CNBr -2 to 6 |
PPCFRSECTEF... |
14.7 |
|
| CNBr 16 |
PRYCLFGNNVT... |
11.2 |
||
| AA 505-599 |
EIAGQVQVDGE... |
10.4 |
||
| CNBr VII |
CNBr -2 and 3 |
PPCFRSECTEF... |
11.7 |
|
| CNBr 8 |
IYIVESSAILFLG... |
12.5 |
||
| CNBr VIII |
CNBr 2 |
PPCFRSECTEF... |
6.2 |
|
| AA 445-480 |
KIVNLLNDLYTR... |
4.3 |
||
| AA 2-69 |
YGFVNHALELL... |
7.7 |
||
| AA 1-69 |
MYGFVNHALEL... |
7.7 |
||
Becker et al. BMC Pharmacology 2001 1:13 doi:10.1186/1471-2210-1-13 |
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