Figure 7.

Coomassie-blue stained Western Blot and autoradiogram of photoaffinity labeled sGC fragments after CNBr digest, SDS-PAGE on a TRIS-Tricin-gel (16.5%) (48 h exposition on Imaging-Plate screened by a folio of contamination monitors). Shown are a ¹⁴C-labeled rainbow molecular weightmarker (¹⁴C-M), further molecular weightmarkers, and photoaffinity labeled sGC (200 μg) after CNBr digest (sGC CNBr). The arrows show the molecular weightmarkers with their molecular weights (kDa) or the ³H-meta-PAL labeled sGC fragments (CNBr VI-VIII) with their molecular weights, as calculated after sequencing.