Figure 5.

Autoradiogram of ³H-meta-PAL labeled sGC after separation by SDS-PAGE. Lane1: 12.5 μg sGC with ³H-meta-PAL without irradiation; Lane 2: 12.5 μg sGC with ³H-meta-PAL after irradiation; Lane 3: as 2 but in the presence of 100 μM unlabeled meta-PAL; Lane 4: as 2 but in the presence of 10 μM unlabeled meta-PAL; Lane 5: as 2 but in the presence of 100 μM ODQ; Lane 6: as 2 but in the presence of 100 μM YC-1.