APP expression in CHO cells. (A) A representative western blot analysis of cellular holo-APP in CHO cells and CHO cells stably transfected with the APP 'Swedish' mutation (CHO/ΔNL). Cellular holo-APP was detected in cell lysates with 22C11 antibody (directed to the APP amino terminus). The loading of the lanes was normalized to levels of β-tubulin. (B) Aβ was detected in the medium of CHO and CHO/ΔNL cells by immunoprecipitation and western blotting with monoclonal antibody 6E10 (which recognizes an epitope within residues 1–17 of Aβ domain). (C) Aβ1–40 and Aβ1–42 levels were measured using standard sandwich ELISA (BioSource, Camarillo, California, USA).
Avramovich-Tirosh et al. BMC Neuroscience 2008 9(Suppl 2):S2 doi:10.1186/1471-2202-9-S2-S2