Additional file 3.
Laminin increases mouse neurosphere formation in an α6β1-dependent manner. (A) Mouse neurospheres were dissociated and plated in medium containing 10 μg/ml laminin ('Medium+Laminin'), resulting in augmented primary neurosphere formation after 7 days in culture. (B) However, this difference was not maintained upon passaging in medium alone (secondary neurosphere formation). (C) Graph demonstrating that the effect of laminin on primary neurosphere formation is mediated by integrin β1. In medium alone, neither an isotype control antibody ('Iso. cont.') nor an integrin β1-blocking antibody ('β1 block'; 1 μg/ml) affected neurosphere formation. Significantly, though, in the presence of laminin the β1 integrin-blocking antibody reduced neurosphere formation to control levels. (D) Similarly, an α6 blocking antibody ('α6 block'; 20 μg/ml) inhibited the effect of laminin ('Laminin') on neurosphere formation, although blocking another laminin-binding integrin, α7 ('α7 block'; 10 μg/ml) did not. ITC, isotype control (A) *p < 0.0001, as determined by Student's t-test; n = 3. (C, D) *p < 0.001, as determined by a one-way ANOVA with Tukey post-test; n = 3.
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Hall et al. BMC Neuroscience 2008 9:71 doi:10.1186/1471-2202-9-71