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Open Access Highly Accessed Research article

Laminin enhances the growth of human neural stem cells in defined culture media

Peter E Hall12, Justin D Lathia123, Maeve A Caldwell24 and Charles ffrench-Constant125*

Author affiliations

1 Department of Pathology, University of Cambridge, Cambridge, UK

2 Cambridge Centre for Brain Repair, University of Cambridge, Cambridge, UK

3 Laboratory of Neurosciences, National Institute on Aging, Baltimore, USA

4 Laboratory for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol, UK

5 MRC Centre for Regenerative Medicine, Queen's Medical Research Institute, Edinburgh, UK

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Citation and License

BMC Neuroscience 2008, 9:71  doi:10.1186/1471-2202-9-71

Published: 23 July 2008

Abstract

Background

Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As such, it is important to identify factors which enhance the growth of hNSC. In vivo, stem cells reside in distinct microenvironments or niches that are responsible for the maintenance of stem cell populations. A common feature of niches is the presence of the extracellular matrix molecule, laminin. Therefore, this study investigated the effect of exogenous laminin on hNSC growth.

Results

To measure hNSC growth, we established culture conditions using B27-supplemented medium that enable neurospheres to grow from human neural cells plated at clonal densities. Limiting dilution assays confirmed that neurospheres were derived from single cells at these densities. Laminin was found to increase hNSC numbers as measured by this neurosphere formation. The effect of laminin was to augment the proliferation/survival of the hNSC, rather than promoting the undifferentiated state. In agreement, apoptosis was reduced in dissociated neurospheres by laminin in an integrin β1-dependent manner.

Conclusion

The addition of laminin to the culture medium enhances the growth of hNSC, and may therefore aid their large-scale production.