Table 1 

Summary table of the observed phenotypes and conductance properties of the N.atKv3.2 single and double S4 mutants 

Channel 
n 
Phenotype 
Delayed Rectifier Properties* 


Wildtype 
8 
Inward rectifier 

H325A 
5 
Delayed rectifier 
V_{50 }= 51.9 ± 3.4 b = 21.8 ± 1.6 
H325C 
5 
Delayed rectifier 
V_{50 }= 62.5 ± 5.7 b = 22.9 ± 2.1 
H325S 
4 
Delayed rectifier 
V_{50 }= 60.6 ± 4.5 b = 19.2 ± 1.9 
H325R 
7 
Delayed rectifier 
V_{50 }= 69.9 ± 8.8 b = 27.3 ± 2.7 
H325E 
18 
Inward rectifier 

H325V 
12 
Inward rectifier 

G331P 
6 
Inward rectifier 

G331A 
14 
Inward rectifier 

G331R 
7 
Delayed rectifier 
V_{50 }= 73.8 ± 9.7 b = 23.8 ± 2.8 
G331K 
12 
Delayed rectifier 
V_{50 }= 36.0 ± 1.0 b = 15.2 ± 0.8 
H325A + G331P 
8 
Inward rectifier 

H325C + G331P 
6 
Inward rectifier 

H325S + G331P 
6 
Inward rectifier 

H325R + G331P 
11 
Inward rectifier 

H325E + G331P 
6 
Inward rectifier 

H325V + G331P 
8 
Inward rectifier 

H325A + G331A 
9 
Inward rectifier 

H325C + G331A 
7 
Inward rectifier 

H325S + G331A 
7 
Inward rectifier 

H325R + G331A 
8 
Inward rectifier 

H325E + G331A 
8 
Inward rectifier 

H325V + G331A 
9 
Inward rectifier 

H325A + G331R 
8 
Inward rectifier 

H325C + G331R 
7 
Inward rectifier 

H325S + G331R 
n/a 
No detectable current 

H325R + G331R 
7 
Inward rectifier 

H325E + G331R 
10 
Inward rectifier 

H325V + G331R 
10 
Inward rectifier 

H325A + G331K 
12 
Combined 
V_{50 }= 22.5 ± 1.8 b = 15.7 ± 1.5 
H325C + G331K 
7 
Combined 
V_{50 }= 15.6 ± 3.4 b = 23.7 ± 2.8 
H325S + G331K 
6 
Combined 
V_{50 }= 7.6 ± 0.4 b = 15.0 ± 0.4 
H325R + G331K 
6 
Delayed rectifier 
V_{50 }= 25.6 ± 1.2 b = 16.5 ± 1.0 
H325E + G331K 
7 
Combined 
V_{50 }= 27.5 ± 2.1 b = 23.3 ± 2.1 
H325V + G331K 
7 
Combined 
V_{50 }= 12.1 ± 1.2 b = 10.2 ± 1.1 


Channels were expressed in X. laevis oocytes and their electrophysiological properties were evaluated. V_{50 }and Boltzmann slope factor were obtained by fitting to the delayed rectifier components of the currentvoltage data. 

Klassen et al. BMC Neuroscience 2008 9:52 doi:10.1186/14712202952 