Table 1

Antibodies used for immunotagging of neuronal nuclei.

Primary/secondary antibody conjugate


anti-NeuN (Ms)

anti-Ms IgG Alexa 488

Blocking Solution

1 × PBS

Nuclei solution


NeuN

1.2 μl

1 μl

100 μl

300 μl

1000 μl

Negative control

0 μl

0.2 μl

20 μl

60 μl

200 μl


Jiang et al. BMC Neuroscience 2008 9:42   doi:10.1186/1471-2202-9-42

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