Open Access Highly Accessed Methodology article

Isolation of neuronal chromatin from brain tissue

Yan Jiang12, Anouch Matevossian1, Hsien-Sung Huang12, Juerg Straubhaar3 and Schahram Akbarian1*

Author Affiliations

1 Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA, USA

2 Graduate School of Biomedical Sciences, University of Massachusetts Medical School, Worcester, MA, USA

3 Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA

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BMC Neuroscience 2008, 9:42  doi:10.1186/1471-2202-9-42

Published: 28 April 2008

Additional files

Additional file 1:

(A) Images from ethidium bromide-stained 1.3% agarose gels showing chromatin DNA from mouse forebrain before (MNase-) and after (MNase+) micrococcal nuclease (MNase) digestion. All samples were treated with RNase A. The DNA ladder is shown on the left side of gel picture. Notice approximately 146 bp DNA fragment only in MNase+ samples. (B), SYBR-green based melting curves from immunoprecipitates with anti-H3K4me2 antibody using primer pairs for mouse B2m and Gad1; notice single peak for specific product. (C) Representative amplification curves of inputs (black circles), immunoprecipitates (red circles) and IgG control (green circles), dotted line indicating cycle threshold. Data shown for Gad1 and B2m separately. Notice samples processed with non-specific IgG show much higher cycle thresholds than input and immunoprecitats.

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