Tmem10 colocalizes with actin in processes and membrane ruffles. (A) Oli-neu cells were transfected with a plasmid encoding for rat Tmem10 and subsequently stained for Tmem10 (green) and Actin (red) with rhodamin-labelled phalloidin. Note the colocalization of Tmem10 with Actin in processes and membrane ruffles. The absence of Tmem10 labelling in untransfected cells shows the specificity of the generated anti-Tmem10 antiserum. (B, C) Oli-neu cells were transfected with Tmem10-EYFP and treated with 2 μM latrunculin A for 30 min 16 h after transfection. (B) FRAP was measured by bleaching a squared region of interest within the cell body and fluorescence recovery in this regions was examined. Average FRAP tracings for 15 cells form 2 independent experiments are shown. (C) Tmem10-EYFP (green) accumulates in intracellular sites (arrow head) after disruption of the F-actin (red) cytoskeleton with latrunculin A. Scale bar, 10 μm.
Kippert et al. BMC Neuroscience 2008 9:40 doi:10.1186/1471-2202-9-40