Figure 3.

Comparative analysis of protein profiles from synaptosomes and crude cytosolic fractions obtained from CA3-CA1 cultured cells. A) Exemplar protein profiles of crude cytosolic (left) and synaptosomes (right) fractions, separated by two-dimensional gel electrophoresis and silver stained. B) Distributions of spot intensities (black histograms) and background noise (grey histograms) from the same 2D gels of panel A from cytosol (upper panel) and synaptosomes (lower panel). C) Digital representation of the superimposition of the two protein profiles (cytosol, blue; synaptosomes, red) after spot detection (see methods for details).