PV-1 mRNA expression in intracranial glioma xenografts. A. RT-PCR for PV-1 was performed on reverse-transcribed RNA harvested from U87MG tumors (T1) and U87:U87/VEGF tumors grown in mice (T2, T3) and compared to PV-1 expression levels in normal mouse brain (NL). Reactions were normalized using primers to the mouse endothelial gene, flk-1. B. Quantitative RT-PCR analysis of PV-1 expression. Reactions were normalized to flk-1. Numerical values for the fold increase are indicated. *Statistically significant difference between the levels of PV-1 expression in NL vs. T2 and NL vs. T3 (p = 0.05).
Shue et al. BMC Neuroscience 2008 9:29 doi:10.1186/1471-2202-9-29