eGFP expression in the brain of a transgenic rat (Ubi-C promoter). Upper panel: eGFP expression is restricted to neurons in transgenic rat brain. A. Confocal microscopy shows extensive eGFP expression in dentate granule cells. B. GFAP immunoreactivity reveals astroglial cells. C. Merged images show a lack of colocalization of eGFP and GFAP signals. Middle panel: confocal images reveal robust eGFP expression in neuronal cell bodies. D. Cortex. E. Striatum. F. CA1 region of hippocampus. G. Hippocampal dentate gyrus. Lower panel: Mixed primary cultures from E18 embryos were stained with anti-beta III tubulin to identify neurons (I; red) and GFAP to label glial cells (J; purple). eGFP expression in cultured neurons was concentrated in the nucleus as well as the cytoplasm (H; green). Arrowheads denote individual neurons in all panels, and the merged image (K) shows eGFP in neurons but not in glial cells.
Agca et al. BMC Neuroscience 2008 9:28 doi:10.1186/1471-2202-9-28