Table 1

T1R & G-protein co-localization in Taste Buds

Immunocytochem.

Single Cell RT-PCR


TrpM5 with

%

TrpM5 with

%

Gq/11/14

14/27

51.9

Gα14

13/21

61.9

Gαgus

17/27

63.0

Gαgus

15/21

71.4

Gq/11/14 + Gαgus

4/27

14.8

Gα14 + Gαgus

7/21

33.3


Gq/11/14 with

Gα14 with

T1R3(GFP)

30/35

85.7

T1R3

13/14

92.9

T1R2

10/14

71.4

T1R3 + T1R2

10/14

71.4


T1R3(GFP)

T1R3+T1R2 with

(includes T1R1 & TT1R2)

Gαq

5/10

50.0

Gq/11/14

30/41

73.1

Gα14

10/10

100.0

Gαgus

11/41

26.8

Gαgus

4/10

40.0


Results from immunocytochemistry are not significantly different (p > 0.05) from data from single cell RT-PCR (Fisher's Exact Test). The immunocytochemical data and RT-PCR data showing co-expression of gustducin with T1Rs is not directly comparable since some cells express only T1R3 and not T1R2+T1R3

Tizzano et al. BMC Neuroscience 2008 9:110   doi:10.1186/1471-2202-9-110

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