Additional file 3.

Gq/11/14 and Gagus staining in T1R3-GFP mouse. Single confocal image planes from the image shown in Fig. 5; T1R3-GFP (B, green), Gq/11/14 (D, red) and Gagus (F, blue), respectively. Co-localization in single plane images of TrpM5-GFP and Gq/11/14 (A), TrpM5-GFP and Gagus (C), Gagus and Gq/11/14 (E) in the vallate papilla. The Gq/11/14 antibody stains most of T1R3-GFP cells (30 of 41). Fewer cells are strongly positive for Gagus (blue). The large majority of Gq/11/14-IR cells (30 of 35) exhibit T1R3-GFP. Only about half of Gagus IR cells show T1R3-GFP expression. Scale bar 20 μM.

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Tizzano et al. BMC Neuroscience 2008 9:110   doi:10.1186/1471-2202-9-110