Additional file 3.
Neurotoxicity of MoPrP105-132 and inhibition by Squalestatin. NB4 neuroblastoma cells were plated at 3 × 104 cells/well into 96-well microtiter plates and allowed to adhere overnight. The following day, cells were treated with (A) either MoPrP105-132 or rhodamine labeled MoPrP105-132 and cell viability determined 24 h later. (B) Alternatively cells were treated for 24 h with 100 nM squalestatin or carrier (DMSO) prior to addition of MoPrP105-132 and incubation for 24 h. Cell viability was determined using the MTT method Optical density was measured at 595 nm, and results calculated by reference to untreated cells.
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Wilson et al. BMC Neuroscience 2007 8:99 doi:10.1186/1471-2202-8-99