Table 1

Previous studies employing microarray approaches to study stroke

Soriano et al. 2000

Jin et al. 2001

Kim et al. 2002

Rao et al. 2002

Schmidt-Kastner et al. 2002

Tang et al. 2002

Roth et al. 2003

Kim et al. 2004

Lu et al. 2004

Moore et al. 2005

Ford et al. 2006

Tang et al. 2006

Vikman and Edvinsson 2006

Our data


Material used

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue

Rat brain tissue



Model of ischemia

Permanent focal MCAO

Transient global MCAO

Permanent focal MCAO

Transient focal MCAO

Transient focal MCAO

Permanent focal MCAO

Permanent focal MCAO

Transient focal MCAO

Transient focal MCAO

Blood from ischemic stroke patients

Permanent and transient focal MCAO

Blood from ischemic stroke patients

Post-mortem brain tissue from 11 stroke patients

Post-mortem brain tissue from 12 stroke patients and permanent focal rat MCAO


No of genes

750

374

1176

1263

9044

~8,000

~13,000

5,000

1,322

~19,000

8784

~39,000

7458

1176


Time after ischemia

3 hours

4 hours

24 hours

72 hours

6 hours

6 hours

24 hours

5 hours

24 hours

1 hours

3 hours

6 hours

24 hours

3 hours

6 hours

12 hours

1 days

2 days

4 days

30 min

4 hours

8 hours

24 hours

3 days

7 days

As soon as possible after hospitalization

24 hours

3 hours

5 hours

24 hours

7–10 days (obtained 2–3 days post-mortem)

1 hour-21 days (rat) and 2–37 days (human, obtained by 6 hours post-mortem)


Cut-off values

2.0-fold

1.7-fold

2.0-fold

2.5-fold

1.7-fold

2.0-fold

3.0-fold

2.0-fold

2.0-fold

-

2.0-fold

1.5-fold

-

2.0-fold


Confirmation of results

In situ hybridization, western blotting

Western blotting, immuno-histochemistry

RT-PCR

Real-time PCR, antisense knockdown, western blotting, immuno-histochemistry

Microarray analysis only

Real-time RT-PCR

Cell culture, in situ hybridization, western blotting, immuno-fluorescence

Cell culture, northern blotting, RT-PCR, western blotting, immuno-histochemistry

Real-time RT-PCR

Real-time RT-PCR

Microarray analysis only

Microarray analysis only

Real-time PCR, immuno-histochemistry

Cell culture, RT-PCR, western blotting, immuno-histochemistry, immuno-fluorescence




Selected molecules

NGFI-C

ARC

GRB2

SMN1

IFN-IP

NDGAP-1

NPR

SOCS-3

NARP

SPR

SPIN2C

ARG1

LBP

PC4

FAK

Synaptic proteins

CD14

CD36

FcGR2A

IFNGR1 caspase-1 a-catenin

LY64

ELK3

POU3F4

RHOA

PAK1

MMP11

INI1


Mitsios et al. BMC Neuroscience 2007 8:93   doi:10.1186/1471-2202-8-93

Open Data