Figure 9.

Amyloid-tau treatment induces rat hippocampal cell apoptosis. Flow cytometric analysis of primary hippocampal cells after treatment with 2 μM formaldehyde-treated tau. The percentage of apoptotic cells were characterized as those that stained with Annexin-V and excluded PI (see Materials and Methods). (A) Control cultured in DMEM without serum. (B), (C) and (D) represent the results of cells exposed to 0.1%-formaldehyde treated amyloid-tau for 24, 48, and 72 h, respectively. (E) and (F) represent cells incubated for 72 h with 0.05%- or 0.01%- formaldehyde-treated tau, respectively. Data represent the mean values of three independent experiments.