Table 2

Summary of validation by WB and immunolocalization

Protein

IPI

Function

ASAP Ratio

WB of mitochondria

WB of cytosol

Co-localized with cyt C*


MAP2K2

00135708

00407256

00231331

Signaling

0

Low sensitivity

Low sensitivity

N/A

Sacsin

00124120

00373012

Folding degradation stability

2.26 ± 0.38

1.77 ± 0.09

0.82 ± 0.02

N/A

SNX1

00198369

00125441

Transport

2.21 ± 0.6

1.51 ± 0.03

Not detected

Yes

SHH

00123314

Signaling

5.47 ± 0.98

Low specificity

Low specificity

Yes

VAMP3

00132276

(00210971)

Transport

0.34 ± 0.07

0.48 ± 0.13

1.16 ± 0.15

N/A


*: Among the five candidate proteins, only antibodies against SHH and SNX1 demonstrated enough sensitivity and specificity for immunocytochemistry.

Jin et al. BMC Neuroscience 2007 8:67   doi:10.1186/1471-2202-8-67

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