Table 1

Mitochondrial proteins affected by rotenone treatment

Increased ≥ two-fold after rotenone treatment


protein

Description

ASAPRatio*

Ref(s) for relevance to neurodegenerative disease


IPI00319790

Dnaj (Hsp40) homolog, subfamily C, member 7

0

IPI00331385

Mdj11

IPI00371053

Similar to dnaj (Hsp40) homolog, subfamily C, member 7

IPI00128647

Crumbs-like protein 1 precursor

0

IPI00204778 (IPI00129395)

Large neutral amino acids transporter small, subunit 1

0

IPI00331577

Solute carrier family 7 (Cationic amino acid transporter y+ system) member 5

IPI00223004

Peroxisomal CA-dependent solute carrier homolog

0

IPI00208799

Solute carrier organic anion transporter family member 1A5

0

IPI00130878

Voltage-dependent T-type calcium channel alpha-1H subunit

0

IPI00388311

Ensembl_locations(Chr-bp):10-14622136

IPI00189595

Voltage-dependent T-type calcium channel alpha-1H subunit

IPI00213584

Alanine – glyoxylate aminotransferase 2 mitochondrial precursor

0

IPI00365283

Similar to capping protein beta subunit, isoform 2

0

IPI00406800

Splice isoform 1 of P47757 F-actin capping protein beta subunit

IPI00113992

GDP-mannose pyrophosphorylase B homolog

0

IPI00153603

28S ribosomal protein s18c, mitochondrial precursor

0

IPI00372556

Similar to NG26

0

IPI00130339

Protein BAT5

IPI00123138

Leucyl-trna synthetase

0

IPI00116966

Asparagine synthetase

0

IPI00406634

Ensembl_locations(Chr-bp):8-124923273

0

[48]

IPI00170128 (IPI00327108)

Paraplegin

IPI00134390

Microsomal dipeptidase precursor

0

IPI00200392

SMHS2

0

IPI00177183

RIKEN cDNA 4833427E09 gene

IPI00116913

Laminin alpha-5 chain precursor

0

IPI00331507

(IPI00325517)

Cullin homolog 5

0

IPI00135708

Dual specificity mitogen-activated protein kinase kinase 2

0

[49]

IPI00407256

Mitogen activated protein kinase kinase 2

IPI00231331

Dual specificity mitogen-activated protein kinase kinase 2

IPI00118384

14-3-3 protein epsilon

0

(IPI00325135)

IPI00309516

Hedgehog-interacting protein

0

IPI00318010

Polycystic kidney disease 1-like 2

0

IPI00368473

Nuclear mitotic apparatus protein 1

0

IPI00371741

Similar to zinc finger protein TZF-L

0

IPI00408182

Tmc3 protein

0

IPI00222063

Weakly similar to hypothetical 71.7 kda protein

0

IPI00222264

HRIHFB2003 protein homolog

0

IPI00366079

Similar to expressed sequence AL022641

0.02

IPI00163011

Thioredoxin domain containing protein 5 precursor

IPI00396730

Growth differentiation factor 10

0.1 ± 0.02

IPI00165799

UBX domain-containing protein 2

0.12 ± 0.1

IPI00371952

Similar to RIKEN cDNA 1300013G12

IPI00129470

Ran gtpase-activating protein 1

0.13 ± 0.03

IPI00319167

Hydrocephalus inducing

0.13 ± 0.05

IPI00310128

Tissue inhibitor of metalloproteinase 2

0.15 ± 0.02

IPI00132801

Glucagon-like peptide 1 receptor precursor

0.22 ± 0.05

IPI00311576

Testis expressed gene 10

0.24 ± 0.08

IPI00400137

RIKEN cDNA 1200011N24

0.27 ± 0.05

IPI00403336

MKIAA0567 protein

IPI00117657

Dynamin-like 120 kda protein, mitochondrial precursor

IPI00341975

RIKEN cDNA 6430598A04 gene

0.27 ± 0.09

IPI00317309

Annexin A5

0.27 ± 0.12

[50]

IPI00231954

6-phosphofructokinase, type C

0.31 ± 0.06

IPI00113824

Basement membrane-specific heparan sulfate proteoglycan core protein precursor

0.33 ± 0.09

IPI00132276 (IPI00210971)

Vesicle-associated membrane protein 3

0.34 ± 0.07

IPI00210089

Voltage-gated sodium channel

0.35 ± 0.06

[51]

IPI00110560

Talin 1

0.36 ± 0.06

IPI00362014

similar to talin

IPI00191107

Similar to mitochondrial ribosomal protein S21

0.37 ± 0.16

IPI00391281

Ensembl_locations(Chr-bp):2-190609002

IPI00127069

Sideroflexin 2

0.38 ± 0.05

[52]

IPI00400079

Splice isoform 1 of Q9JLT4 Thioredoxin reductase 2, mitochondrial precursor

0.4 ± 0.19

IPI00124699

Thioredoxin reductase 2

IPI00403407

Adult male stomach cDNA, RIKEN full-length enriched library, clone:2210009O12 product:thioredoxin reductase 2, full insert sequence

IPI00350590

Splice isoform 4 of Q9JLT4 Thioredoxin reductase 2, mitochondrial precursor

IPI00207072

NADH-ubiquinone oxidoreductase 13 kda-A subunit, mitochondrial precursor

0.41 ± 0.13

IPI00330862

Villin 2

0.42 ± 0.07

IPI00111460

Williams-Beuren syndrome chromosome region 16 protein homolog

0.46 ± 0.12

IPI00189798

Similar to Williams-Beuren syndrome chromosome region 16 homolog

IPI00112935

Serine hydroxymethyltransferase

0.47 ± 0.06

[53]

IPI00131577

Heme oxygenase 1

0.47 ± 0.12

[54]

IPI00177038

Actin-like protein 2

0.47 ± 0.17

IPI00400012

Similar to Actin-like protein 2 (Actin-related protein 2)

IPI00362072

Similar to actin-related protein 2

IPI00364385

Similar to RIKEN cDNA 5730406I15

0.5 ± 0.19

IPI00228236

Microsomal signal peptidase 25 kda subunit


Decreased ≥ two-fold after rotenone treatment


IPI00212082

Ensembl_locations(Chr-bp):1-153887103

2.03 ± 0.62

IPI00368435

Similar to Ten-m4

IPI00157497

Ten-m4

IPI00351867

Odd Oz\ten-m homolog 4

IPI00210503

Long-chain-fatty-acid – coa ligase 4

2.04 ± 0.44

IPI00403180

(IPI00378474)

Similar to mitochondrial isoleucine trna synthetase

2.06 ± 0.22

IPI00408243

Similar to single-stranded DNA binding protein

2.07 ± 0.19

IPI00124980

Prolactin regulatory element-binding protein

2.09 ± 0.48

IPI00229040

Retinol dehydrogenase 13

2.15 ± 2.41

IPI00198369

Sorting nexin 1

2.21 ± 0.6

IPI00125441

Sorting nexin 1

IPI00124120

Sacsin

2.26 ± 0.38

IPI00373012

Similar to sacsin

IPI00364603

Similar to RNA-binding protein EWS

2.33 ± 0.31

IPI00191745

Similar to coproporphyrinogen oxidase

2.38 ± 0.41

IPI00192034

(IPI00114375)

Dihydropyrimidinase related protein-2

2.46 ± 0.44

IPI00285485

Protein kinase C-binding protein NELL2 precursor

2.57 ± 0.61

IPI00136067

Jagged 2 precursor

2.73 ± 0.46

IPI00365920

Jagged 2

IPI00311509

Aladin

2.9 ± 0.64

IPI00205182

Interleukin-6 receptor alpha chain precursor

3.03 ± 0.48

IPI00206288

interleukin 6 receptor

IPI00344360

Transcription termination factor, mitochondrial

4 ± 1.42

[55]

IPI00370029

Similar to NG28

4.43 ± 0.97

IPI00378753

Similar to aminopeptidase N

4.68 ± 0.99

IPI00405386

T-complex protein 10a

5.36 ± 0.87

IPI00230453

Similar to VIP36-like protein precursor (Lectin, mannose-binding 2-like)

5.45 ± 0.6

IPI00357887

Similar to lectin, mannose-binding 2-like

IPI00123314

Sonic hedgehog protein precursor

5.47 ± 0.98

[56]

IPI00196054

(IPI00125256)

C-C chemokine receptor type 2

6.4 ± 1.67

IPI00353420

Cytoskeletal protein

7.56 ± 1.41

IPI00388450

Protein tyrosine phosphatase, receptor type, U

8 ± 1.03

IPI00338565

Mutant fibrillin-1

8.39 ± 2.97

IPI00122438

Fibrillin 1 precursor

IPI00229434

Apoptosis stimulating of p53 protein 2

38.26 ± 2.99

IPI00269076

Adenylate kinase 2

999

IPI00214038

(IPI00118120)

Myosin Va

999

IPI00393437

Ensembl_locations(Chr-bp):8-80002855

IPI00405881

Ensembl_locations(Chr-bp):9-77611564

IPI00331016

Similar to SEC24 related gene family, member B

999

IPI00372727

Similar to Protein transport protein Sec24B (SEC24-related protein B)

IPI00309437

ALDR protein

999

IPI00387535

Fatty acid coenzyme A ligase, long chain 3

999

IPI00169772

Long-chain-fatty-acid – coA ligase 3

IPI00330207

Splicing factor 3B subunit 1

999

[57]

IPI00366952

Splicing factor 3b, subunit 1, 155kd

IPI00118235

Mitochondrial 60S ribosomal protein L3

999

IPI00115094

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor

999

IPI00409229

Ensembl_locations(Chr-bp):7-120047962

IPI00204344

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor

IPI00324180

Breast cancer type 2 susceptibility protein homolog

999

IPI00366614

Breast cancer susceptibility protein BRCA2

IPI00118021

Gtrgeo22

999

IPI00313998

Sulfide:quinone oxidoreductase, mitochondrial precursor

999

IPI00357889

Similar to 28S ribosomal protein S9, mitochondrial precursor (MRP-S9)

999

IPI00132700

39S ribosomal protein L35, mitochondrial precursor

999

IPI00189766

Membrane associated progesterone receptor component 1

999

IPI00208648

Disks large-associated protein 2

999

IPI00117912

Semaphorin 5B precursor

999

IPI00400269

Hypothetical protein

IPI00365296

Similar to sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B

IPI00403907

Sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain

IPI00127131

Osa1 nuclear protein

999

IPI00113214

Ubiquitin carboxyl-terminal hydrolase 5

999

[58]

IPI00118333

RW1 protein

999

IPI00362105

Similar to proteasome 26S ATPase subunit 6

999

IPI00125971

26S protease regulatory subunit S10B

IPI00231757

Proteasome (prosome, macropain) subunit, alpha type 2

999

[59]

IPI00404117

Proteasome subunit alpha type 2

IPI00318970

Proteasome (Prosome, macropain) subunit, alpha type 2

IPI00337930

RIKEN cDNA 4930432B04

999

IPI00203604

Brain protein 44-like protein

999

IPI00402961

Ensembl_locations(Chr-bp):12-106426680

IPI00124292

Brain protein 44-like protein

IPI00406403

Ensembl_locations(Chr-bp):Un_random_NT_060620-5611

999

IPI00381837

similar to putative pheromone receptor

IPI00372549

Similar to MHC class I cell surface glycoprotein

999

IPI00367751

Similar to pecanex 1

999

IPI00365997

Similar to PHD finger protein 2

999

IPI00114424

RIKEN cDNA D930036F22 gene

999

IPI00326141

Augmenter of liver regeneration

999

IPI00191045

Similar to zinc finger protein 296

999

IPI00210183

Nuclear receptor binding factor-1

999


IPI: International protein index. ASAP Ratio: Automated Statistical Analysis of Protein abundance ratio. The results are expressed as 12C (DMSO)/13C (Rotenone). *: Ratio was expressed as 0 and 999 when only the 13C- and 12C-labeled peptides were found, respectively, in the sample. Proteins sharing the same peptides but with different protein identification numbers are listed in one cell.

Jin et al. BMC Neuroscience 2007 8:67   doi:10.1186/1471-2202-8-67

Open Data