Validation of rotenone-affected mitochondrial proteins by WB. Purified mitochondria were isolated from MES cells treated with 20 nM rotenone or vehicle (DMSO), respectively. Equal amounts of mitochondrial proteins (20 μg) were loaded onto an 8–16% or 4–15% SDS-PAGE and analyzed by WB. The WB ratio in mitochondria and cytoplasm was calculated as DMSO/rotenone (D/R) from 3 batches of independent experiments and is shown in Table 2.
Jin et al. BMC Neuroscience 2007 8:67 doi:10.1186/1471-2202-8-67