Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells
1 Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA
2 Department of Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
BMC Neuroscience 2007, 8:67 doi:10.1186/1471-2202-8-67Published: 16 August 2007
Many studies have shown that mitochondrial dysfunction, complex I inhibition in particular, is involved in the pathogenesis of Parkinson's disease (PD). Rotenone, a specific inhibitor of mitochondrial complex I, has been shown to produce neurodegeneration in rats as well as in many cellular models that closely resemble PD. However, the mechanisms through which complex I dysfunction might produce neurotoxicity are as yet unknown. A comprehensive analysis of the mitochondrial protein expression profile affected by rotenone can provide important insight into the role of mitochondrial dysfunction in PD.
Here, we present our findings using a recently developed proteomic technology called SILAC (
Our findings represent the first report of these mitochondrial proteins affected by rotenone; further characterization of these proteins may shed more light on PD pathogenesis.