Intercellular trafficking of VP22-MICDYS in the transfected C2C12 cells. 3T3 cells were transfected with the plasmid pAMICDYS (A), pAVP22-MICDYS (B), pAVP22 (C) respectively. 48h post-transfection, transfected cells were fixed and immunocytochemistry was performed with the monoclonal anti-dystrophin Amino Terminal. Magnification: × 100. At 48 h after transfection with pAVP22, pAMICDYS, pAVP22-MICDYS, cell supernatant were prepared from 3T3 cells and then added to C2C12 cells in a 35 mm dish. After 45 min, the cells were washed with PBS and processed for immunocytochemistry. (D) Transfected by supernatant with pAMICDYS; (E) Transfected by supernatant with pAVP22-MICDYS. Magnification: × 200.
Xiong et al. BMC Neuroscience 2007 8:50 doi:10.1186/1471-2202-8-50