Table 1

Summary of antibodies tested on Western Blot
Antibody
 Overexpressed mLRRK2
 Overexpressed hLRRK2
 Endogenous mLRRK2
 Endogenous hLRRK2
 Peptide Location
Abgent AP7099a
 -
 -
 -
 -
 N/A
Abgent AP7099b
 -
 -
 -
 -
 (LRR, AA 1246–1265, 100% identity)
H: WSRVEKLHLSHNKLKEIPPE
M: WSRVEKLHLSHNKLKEIPPE
Abgent AP7099c
 -
 -
 -
 -
 (N-Term, around L229)
H: EEIVLHVLHCLHSLAIPCNNVEVLMSGNVR
M: KEIVYHVLCCLHSLAVTCSNVEVLMSGNVR
Abgent AP7099d
 -
 -
 -
 -
 (N-Term, around E285)
H: VSCCLLHRLTLGNFFNILVLNEVHEFVVKA
M: VSCSLFQKLTLGNFFNILVLNEVHVFVVKA
Abgent AP7099e
 -
 +
 -
 +
 (N-Term, around E519)
H: RAILHFIVPGMPEESREDTEFHHKLNMVKK
M: RAILHFVVPGLLEESRE..DSQCRPNVLRK
Abgent AP7099f
 -
 +
 -
 -
 (N-Term, around L893)
H: AQSDDLDSEGSEGSFLVKKKSNSISVGEFY
M: GQSDDLDSEGSESSFLVKRKSNSISVGEVY
Abgent AP7099g
 -
 +
 -
 -
 (N-Term, around L899)
H: AQSDDLDSEGSEGSFLVKKKSNSISVGEFY
M: GQSDDLDSEGSESSFLVKRKSNSISVGEVY
Abgent AP7099h
 -
 +
 -
 -
 (N-Term, around L955)
H: IFDHEDLLKRKRKILSSDDSLRSSKLQSHM
M: VFDHEDLLRRKRKILSSDESLRSSRLPSHM
Abgent AP7099i
 -
 -
 -
 -
 (C-Term, around C2354)
H: STERNVMWGGCGTKIFSFSNDFTIQKLIET
M: SSERHITWGGCGTKVFSFSNDFTIQKLIET
Abgent AP7099j
 -
 -
 -
 -
 (C-Term, around K2402)
H: CVHFLREVMVKENKESKHKMSYSGRVKTLC
M: CVHFLKEVMVKLNKESKHQLSYSGRVKALC
Abgent AP7099k
 -
 -
 -
 -
 (C-Term, around Y2475)
H: MTAQLGSLKNVMLVLGYNRKNTEGTQKQKE
M: ATAQLGSLKNVMLVLGYKRKSTEGIQEQKE
Abgent AM7099a
 -
 -
 -
 -
 (monoclonal) N/A
Abgent AM7099b
 -
 -
 -
 -
 (monoclonal) N/A
Abcam 27482
 -
 +
 -
 -
 N/A
Abcam 19906
 -
 -
 -
 -
 N/A
Novus Biological 267
 -
 +
 -
 +
 (N-Term, AA 920–945)
H: SNSISVGEFYRDAVLQRCSPNLQRHS
M: SNSISVGEVYRDLALQRYSPNAQRHS
Novus Biological 268
 -
 +
 -
 +
 (C-Term, AA 2500–2527)
H: INLPHEVQNLEKHIEVRKELAEKMRRTSVE
M: LNLPHEVQNLEKHIEVRTELADKMRKTSVE
Chemicon AB9682
 -
 +
 -
 -
 N/A, synthetic peptide from rat
Chemicon AB9704
 -
 +
 -
 -
 N/A
Alexis (AT106)
 +
 +
 +
 +
 (AA 1838–2133)
Cell Signaling 2567
 +
 +
 -
 -
 (C-Term, G2090)
H: FPNEFDELEIQGKLPDPVKEYGCAPWPMVE
M: FPNEFDELAIQGKLPDPVKEYGCAPWPMVE
JH5514
 +
 +
 +
 +
 (C-Term, AA 2500–2515), 100% identity
H: INLPHEVQNLEKHIE
M: LNLPHEVQNLEKHIE

Summary of the results of commercially available antibodies against LRRK2 tested on Western Blot. A (+) denotes antibodies that produce a discernable band specific to LRRK2 protein. A (-) denotes antibodies unable to detect a band specific to LRRK2 protein. Antibodies were designed against the human sequence unless otherwise indicated.

Biskup et al. BMC Neuroscience 2007 8:102   doi:10.1186/1471-2202-8-102