Figure 4.

Evaluation of the effects of berberine chloride on the mRNA of IL-1β and iNOS by Real time PCR. (A)Real time PCR results of berberine chloride on the IL-1β mRNA indicate that IL-1β mRNA level was increased in the Aβ(1–40) group and Aβ(1–40)+berberine group. The result represents at least three independent experiments, and β-actin was used as an internal control.(B) Real time PCR results of berberine chloride on the iNOS mRNA indicates that iNOS mRNA level was increased in the Aβ(1–40) group and Aβ(1–40)+berberine group. The result represents at least three independent experiments, and β-actin was used as an internal control. Values represent the means ± SD of 6 rats. *P < 0.01 when compared with the normal group. # P < 0.01 when compared with the Aβ(1–40) injection group.