Syntaxin 2 is expressed in GABAergic and glycinergic amacrine cells. A–C: Labeling for syntaxin 2 colocalizes with labeling for GAD-65 in numerous GABAergic amacrine cell bodies (*) in the inner nuclear layer (INL). However, syntaxin 2 labeling is not present in all GAD-65-positive amacrine cells (arrows) and many syntaxin 2-positive amacrine cell bodies do not show labeling for GAD-65 (arrowheads). Images shown from a projection of 12 optical sections with total thickness of 2.78 μm. D–F. At high magnification, labeling for syntaxin 2 in the inner plexiform layer (IPL) shows only limited colocalization with GAD-65 positive presynaptic terminals (circles). Images shown from a projection of 13 optical sections with total thickness of 1.75 μm. G–I: Glycinergic amacrine cells in the INL, labeled for GlyT1, show labeling for syntaxin 2 (*). However, many syntaxin 2-positive amacrine cell bodies are present that do not show labeling for GlyT1 (arrowheads). Images shown from a single optical plane. J–L: At high magnification, syntaxin 2 shows colocalization with GlyT1-positive amacrine cell processes in the IPL (circles) although colocalization is imperfect. Images shown from a projection of 6 optical sections with total thickness of 0.73 μm. GCL; ganglion cell layer. Scale bars= 20 μm for A–C and G–I; 10 μm for D–F; 5 μm for J–L.
Sherry et al. BMC Neuroscience 2006 7:54 doi:10.1186/1471-2202-7-54