Effect of glutamate on LDH release and AIF protein levels in subcellular fractions in HT22 cells and primary cortical cells. A: primary cortical cells were isolated as described under Figure 11 and were treated with 1 mM glutamate at the indicated times. B: HT22 cells were treated with 7–8 mM glutamate at the indicated times. A, B: (I): cell death were measured by LDH release assay. Data are the mean ± SEM values from at least three experiments *P < 0.05 compared to control; A, B: (II): subcellular proteins were isolated from cytosol, nuclei, mitochondria and whole cells as described under "Methods", and then AIF protein levels were detected by Western blot analysis. Actin was used as a loading control.
Zhang and Bhavnani BMC Neuroscience 2006 7:49 doi:10.1186/1471-2202-7-49