Spinal cord histopathology following I/R. Rabbits were treated with vehicle (saline) or TMP (30 mg/kg) prior to the onset of ischemia (30 min), followed by 48 h of reperfusion. Sham animals received the same pretreatment as I/R group, followed by sham operation. The ischemic spinal cord sections were prepared and stained with H&E. Figure A represent the sham rabbits, which show normal histology. Figure B represent control rabbits, which show a pattern of necrotic changes with karyolysis and neurophil vacuolation. Figure C represent TMP- treated rabbits, which show mild degrees of destruction such as triangular shape, and Nissl granule loss in some motor neurons. Figures(magnification × 200) are representative of 3 separate experiments with similar results.
Fan et al. BMC Neuroscience 2006 7:48 doi:10.1186/1471-2202-7-48