Figure 2.

SDF-1 Antibody Specificity. The polyclonal SDF-1 antibody was tested for non-SDF-1 antigenic interactions and non-specific tissue binding. Panel A demonstrates the SDF-1 antibody's recognition of SDF-1 antigen on mouse hippocampus. Panel B demonstrates that preabsorption of the SDF-1 antibody with recombinant murine SDF-1α completely blocks specific binding of the antibody to the tissue with only very low (diffuse background) non-specific binding. Panel C shows that leaving the primary (SDF-1) antibody incubation step out of the staining protocol does not result in non-specific binding of the secondary antibody or the detection reagents. Scale bar equals 200 μm.