Scavenging effect of glycitein in the transgenic C. elegans and in vitro. A. H2O2 level in transgenic C. elegans CL4176 fed with different isoflavones. C. elegans strain CL4176 was maintained at 16°C for 38 h and then temperature up-shifted to 23°C for 48 h, followed by measurement of H2O2 (DCF assay described in methods). CL4176 worms were fed vehicle (Ctrl), 100 μg/ml daidzein, genistein or glycitein from 1 day of age until 3 days of age. At least 60 worms from each group were analyzed for levels of H2O2. Results are expressed as a percentage of fluorescence (%DCF) relative to control. B. Scavenging effect of glycitein on hydroxyl radicals in vitro. The ESR conditions: X-band, 100 kHz modulation with amplitude 1 G, microwave power 10 mW, central magnetic field 3,250 G, sweep width 200 G, temperature 20°C. Inset: ESR spectrum of DMPO-OH generated from Fenton reaction and trapped by DMPO. C. Scavenging effect of glycitein on superoxide radicals in the system. The ESR conditions are the same as in Fig. 3B. Inset: ESR spectrum of DMPO-OOH generated from Xanthine/xanthine oxidase and trapped by DMPO; D. Scavenging effect of Soy isoflavone glycitein on .CH3 free radicals in the in vitro system. Inset: ESR spectrum of CH3-tNB generated from the oxidation of DMSO by ONOO-and trapped by tNB.
Gutierrez-Zepeda et al. BMC Neuroscience 2005 6:54 doi:10.1186/1471-2202-6-54