lin-12 likely acts in a subset of glr-1 expressing neurons to regulate reversals. A. RNAi driven by the glr-1 promoter expressing dsRNA does not spread to nearby tissues. The glr-1 promoter drives expression in the command interneurons AVA, AVB, AVD, AVE, and PVC, as well as AIB, AVG, AVJ, DVC, PVQ, RIG, RIM, RIS, RMD, RMEL/R, SMD, and URY, all of which (except DVC, PVQ, and PVC) are located in the head 48, 49. The lin-12 cDNA fragment used to express lin-12 dsRNA also contains GFP sequences in cis. The dsRNA expressing construct was introduced into strains expressing osm-10p::gfp in ASH neurons or elt-2p::gfp in intestinal cells, and compared to control strains for GFP expression levels. Adult animals from multiple transgenic lines were scored; representative images are shown. B. Effect of altering lin-12 activity in glr-1 expressing neurons. glr-1p indicates the glr-1 promoter used to drive expression of various transgenes, and glr-1p::(0) indicates the promoter only control. lin-12(RNAi) and gfp(RNAi) indicate lin-12 and gfp dsRNA, respectively. lin-12(OE) indicates transgenic animals injected with a rescuing lin-12 cDNA construct at a high concentration (see Methods). lin-12IC indicates a truncated, activated lin-12 allele that lacks the extracellular domain.**p<0.01 and ***p<0.001 vs. wild type, respectively. C. Expressing lin-12 cDNA in glr-1 expressing neurons rescues the lin-12(lf) reversal defect. *** p<0.001.
Chao et al. BMC Neuroscience 2005 6:45 doi:10.1186/1471-2202-6-45