Table 2

Quantitative evaluation of the nature of SON proliferative cells

% of BrdU-labeled nuclei associated with specific cell markers


Cell type

Marker


Neurons

VP + OT

0

Astrocytes

GFAP

6

Oligo. Precursors

NG2

18

Microglia

IsoB4

8

Vessels

IsoB4

60

Vessels

Nestin

67

Vessels

EBA

55


BrdU was administrated to osmotically stimulated rats drinking 2% saline during 6 days, and the animals were fixed 5 hours after the BrdU administration. The relative proportion of each BrdU-labeled cell type was calculated from at least 4 sections per rat in 4 stimulated rats. More than 500 BrdU-labeled nuclei were scored for most markers.

GFAP: glial fibrillary acidic protein; EBA: endothelial brain antigen; NG2: proteoglycan NG2; OT: oxytocin; VP: vasopressin.

Alonso et al. BMC Neuroscience 2005 6:20   doi:10.1186/1471-2202-6-20

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