Table 1

Antibodies used

Marker

Species

Working dilution

Vendor or productor


Proliferation

BrdU

Mouse IgG

1:500

Novo Castra (Newcastle, UK)

BrdU

Rat IgG

1:500

Accurate Chemicals (New York, USA)

Magnocellular neurons

OT

Rabbit IgG

1:2000

Produced by G. Alonso

VP

Rabbit IgG

1:2000

Produced by G. Alonso

Astrocytes

GFAP

Rabbit IgG

1:5000

Dakko (Glostrup, Denmark)

Microglia and endothelial cells

Lectin from bandeirae

1:2000

Sigma

Simplifolia (IsoB4)

Oligodendrocyte progenitors

NG2

Rabbit IgG

1:500

Chemicon (Temecula, USA)

Vessels

Rat IgG-Alexa 488 conjugated

Goat IgG

1:2000

Molecular Probes (Eugene, USA)

EBA

Mouse IGM

1:5000

Sternberger Monoclonals (Lutherville, USA)

Nestin

Mouse IgG

1:100

Hybridoma Bank (Iowa city, USA)

Vimentin

Mouse IgG

1:5000

Sigma


BrdU: bromodeoxy uridine; EBA: endothelial brain antigen; GFAP: glial fibrillary acidic protein; IgG-IgM: immunoglobulins type G and M; OT: oxytocin; VP: vasopressin.

Alonso et al. BMC Neuroscience 2005 6:20   doi:10.1186/1471-2202-6-20

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