Figure 2.

Changes in phosphorylation of PKB, GSK3 and MAPK by inhibition of PI3K Rat ARC wedges were pretreated with 10 μM LY294002 or aCSF for 20 minutes and incubated for 1 minute with 10 nM leptin or 1 nM insulin or aCSF. Equal amounts of protein lysate were subjected to SDS-PAGE and transferred to nitrocellulose membrane. The phosphorylated levels of PKB, and GSK3αβ (A) and p42/p44 MAPK (B) were detected by immunoblotting with appropriate specific antibodies. The total amount of PKB is also shown. Bands were quantified using a densitometer. The values are expressed as relative to the corresponding aCSF control group, and normalized for protein loading. Values represent the mean ± SEM for between 4–5 and 3–5 animals for each time point with leptin and insulin respectively. * P < 0.05 and ** P < 0.01.

Mirshamsi et al. BMC Neuroscience 2004 5:54   doi:10.1186/1471-2202-5-54
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