Figure 7.

NT secretion following exposure to and recovery from Oxygen-Glucose Deprivation. Lamina cribrosa cells and ONH astrocytes were exposed to OGD for 48 hours (OGD) or to 48 hours OGD followed by recovery for 24 hours (OGD+R). NGF and BDNF secretion was determined by immunoassay of concentrated conditioned media from three LC cell lines and three ONH astrocyte cell lines. A NT standard was included in each assay and was used to generate a standard curve. Data are reported as percent of the control ± SEM (n = 3). * indicates p < 0.016 for OGD compared to C and OGD+R; † indicates p < 0.016 for OGD+R compared to C (one way analysis of variance [ANOVA] followed by validation using Student-Newman-Keuls tests).