Competition with Aβ1-42 against the increased antibody binding found after acid dissociation of sera from APP mice vaccinated with Aβ. Sera were collected from transgenic mice, diluted 1:1000 and preincubated at either pH 7 (open bars) or pH 2.5 (solid bars) as described in methods. After centrifugation and reconstitution of serum volume, the sera were incubated with Aβ1-42 at the indicated concentrations. Because the Aβ was initially dissolved in DMSO, some samples were also incubated with DMSO at the concentrations found in the 100 μg/ml Aβ incubation (2% DMSO). ELISA assays were completed as described in Methods and the optical density at 450 nm used to estimate the amount of antibody binding to the ELISA plate. Results are presented as mean ± sem.
Li et al. BMC Neuroscience 2004 5:21 doi:10.1186/1471-2202-5-21