Nontransgenic mice immunized with Aβ vaccines do not have increased titers when incubated at pH 2.5. Results are from sera obtained 14 days after the fourth vaccination. Aliquots from each sample were incubated at either pH 2.5 or pH 7.0 for 20 minutes, separated by centrifugation through 10,000 MW filters and brought to neutral pH for standard ELISA assay. Data presented are mean ± sem. Sample size is 5–6 per group.
Li et al. BMC Neuroscience 2004 5:21 doi:10.1186/1471-2202-5-21