Table 2

Secondary antibodies and probes used for immunofluorescence and western blot analysis.

Antibody/Probe (Catalog #)

Conjugate

Dilution

Supplier

Goat anti-mouse IgG (#A-11001)

Alexa-fluor 488

1:200 in PBS

Molecular Probes, Eugene, OR

Goat anti-rabbit IgG (#T-6778)

Alexa-fluor 488

1:200 in PBS

Molecular Probes, Eugene, OR

Goat anti-mouse polyvalent (#A-0162)

Alkaline Phosphatase

1:4000 in PBS

Sigma Chem. Co, St. Louis, MO

Goat anti-rabbit IgG (#A-3812)

Alkaline Phosphatase

1:30,000 in PBS

Sigma Chem. Co., St. Louis, MO

Nucleic Acid binding probe; TO-PRO-3 iodine (#T-3605)

---

1:1000 in PBS

Molecular Probes, Eugene, OR

Weigum et al. BMC Neuroscience 2003 4:6   doi:10.1186/1471-2202-4-6