Figure 2.

Glutamate-induced toxicity in mouse hippocampal HT22 cells as assessed by phase contrast microscopy (200×). HT22 cells were cultured and treated with glutamate alone or in the presence of estrogens for 8 to 18 h. Degenerated cells are depicted by arrows. A = Untreated control cells; B = 10 mM glutamate; C = 10 mM glutamate + 10 μM Δ⁸,17β-E₂; D = 8 mM glutamate + 10 μM 17β-E₂; E = cells treated with 5 mM glutamate for 18 h; F = 5 mM glutamate + 10 μM 17β-E₂ for 18 h.