Figure 1.

Controlled early differentiation of human ES cell colonies (A) Undifferentiated HESC colony on mouse embryonic fibroblasts. (B) Crater containing colony derived from passaging HESC onto freshly plated mouse embryonic fibroblasts. A central crater of monolayer cells (arrowhead) is surrounded by undifferentiated HESC (*), and differentiating cells in an outer ring adjacent to the feeders (arrow). (C,D) OCT-4 expression in undifferentiated HESC (C) and crater cells (D). (E,F) DAPI staining of the same fields as (C,D). (G,H) merged images of (C-F). Mitotic cells (arrowheads) and some of the apparently apoptotic cells (white dots) are indicated. (I) Anti-SSEA-4 immunostaining of a crater colony. Crater cells (arrowhead) do not stain for the SSEA-4 antigen, a marker of undifferentiated HESC, while the surrounding HESC are SSEA-4+ (arrow). Images and scale bars: (A,B) 10×, 100 μm. (C-H) 40×, 50 μm. (I) 10×, 100 μm.

Schulz et al. BMC Neuroscience 2003 4:27   doi:10.1186/1471-2202-4-27
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