Figure 10.

Vacuoles contain focal SOD1 aggregates. Panels A-C are 3-D reconstructed images of a vacuole from SOD1^G93A mouse spinal cord sections stained with anti-SOD1 antibodies (red). The vacuole is bounded by SOD1 and contains many SOD1 aggregates (arrowheads). Panel D is an immunogold electron microscopic image from SOD1^G93A mouse spinal cord stained with an anti-SOD1 antibody (5 nm gold) and an anti-cytochrome oxidase subunit 4 antibody (10 nm gold). The arrowhead indicates a region of SOD1 aggregation.