The 200 kDa band represents a dimer of VR1 subunits. Western blot of oocytes expressing wild-type VR1, Δ2–52, or wild-type VR1 + Δ2–52. Oocytes were prepared as described in Experimental Procedures. The band observed for VR1-express ing oocytes was at 200 kDa, the band observed for the Δ2–52-expressing oocytes was at 180 kDa, while oocytes injected with a 1:1 ratio of VR1 and Δ2–52 RNA yield three bands of 200 kDa, 180 kDa, and 190 kDa. This blot was probed with the FLAG antibody.
Rosenbaum et al. BMC Neuroscience 2002 3:4 doi:10.1186/1471-2202-3-4