Figure 4.

Antisera, raised against Drosophila huntingtin, detected increased signals in the nucleus of S2 cells with nuclear dorsal accumulation after LPS treatment. a,b, Western blot analysis was performed using transfected and non-transfected S2 cells. Full-length and amino-terminal fragment of Drosophila huntingtin were expressed by Gal4-UAS system [31] using pUAS-DhdcDNA (lanes 3 and 5), pUAS-Dhdminigene (lane 7), pUAS-N605 (lanes 1 and 6), and actin5C-Gal4. Antisera 1893 (a) and 1894 (b), raised against the amino-terminal region of Drosophila huntingtin, recognized the products of the transgenes (arrow: full-length huntingtin, and arrow head: N605), and also detected an approximately 400kDa native protein corresponding to over-expressed full-length Drosophila huntingtin in size which is thought to be native Drosophila huntingtin. c, Antiserum 1894 recognized mainly cytoplasmic immunoreactivity with minor nuclear signals. Transfected NLS-GFP-lacZ detected by anti-lacZ antibody and Alexa Fluor 488 goat anti-mouse IgG was used for a control nuclear maker. d, Antiserum1894 recognized apparent nuclear immunoreactivity, when FLAG-Dorsal was accumulated in the nucleus after LPS treatment. FLAG-Dorsal was detected by anti-FLAG M5 antibody and Alexa Fluor 488 goat anti-mouse IgG.

Takano and Gusella BMC Neuroscience 2002 3:15   doi:10.1186/1471-2202-3-15
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