Figure 6.

The specificity of the BMP-5 Ab used in these studies as assessed by Western blot analysis. BMP-5 Ab (0.5 μg/ml) reacts with purified recombinant BMP-5 (50 ng), but not with equal amounts of purified recombinant BMP-6 or -7. Preincubation of BMP-5 Ab with specific blocking peptide (2.5 μg/ml) significantly reduces binding to BMP-5 protein. Data represented in this figure were obtained from the same immunoblot.