Effects of OGD (2 h) followed by reoxygenation (6–24 h) on mitochondrial donut formation. Both XY and XX CGNs were labeled with mitochondrial dye MitoTracker Red CMXRos for the visualization of mitochondrial morphology under fluorescence microscopy. A) Control cells showed tubular and long mitochondria (green arrows) in contrast to those exposed to reoxygenation for 6, 12 and 24 h which showed increased numbers of donut shaped mitochondria showing a central hole (yellow arrows; visualized at 100 X magnification). Scale bar 10 μm. (B) Quantitation of the number of donuts per cell showed increased number of donuts are present in the XY neurons in comparison to that in XX CGNs. Results expressed as mean ± SD (n = 3, *p < 0.05, XY vs. XX, +p < 0.05, ++p < 0.01 vs. control, ANOVA followed by Bonferroni/Dunn post hoc test).
Sharma et al. BMC Neuroscience 2014 15:9 doi:10.1186/1471-2202-15-9